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Experimental Design:

Plots grown from seed and grown as spaced, single plants in the field

  

 





Reciprocal garden transplant sites of single, spaced plants. Red=hays ecotype, green=manhattan ecotype, blue=Illinois ecotype,  Note Colby KS satellite site to test the tolerance of all ecotypes in the driest, western most environment.  The three ecotypes are represented by four populations. These populations were derived from the same source populations as the seeded plots. At each site, there are 20 blocks of the above design at each planting site. Each plot is either 4x4m, or 4x8 meters in area. (Treatments randomized among blocks)

Source populations for the ecotype project:

       Seeds collected from natural field populations (never restored). Four populations per  ecoregion  were collected. The regions were Hays (western KS), Manhattan (eastern KS) and Illinois (carbondale)

Response variables:

    Differential canopy area using imageJ

    Photosynthesis, WUE, SPAD

    Biomass

    Phenology, Plant Height       

    Genetic diversity of seed populations

    Virus and Fungi sorting by ecotype

  

Dots indicate reciprocal garden sites. West to east, Colby, Hays, Manhattan, Illinois.

Differential canopy cover across ecotypes and sites